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. 2014 Oct 24;4:6760. doi: 10.1038/srep06760

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(a) Purified GLUT-1 and Nb-GLUT-1 were quantified by SDS-PAGE against bovine serum albumin (BSA) standards. (b) SPR was used to assess antigen-Nb binding. GLUT-1 or control detergent-buffer solution was immobilized on an amine-coupling sensor chip, and Nb-GLUT-1 was flown as the analyte. Data shown are double-referenced, and are from an experiment that was replicated 4 times with independent batches of purified proteins. (c) HRP-conjugated protein-A antibody directly detects (Left) Nb-GLUT-1, but not (Middle) GLUT-1 on a western blot. Right - Lower µg amounts of GLUT-1 were successfully detected on a western blot using Nb-GLUT-1 as the primary antibody, followed by HRP-conjugated protein-A as the secondary antibody. Data shown have been replicated 5 times with independent batches of purified proteins.