Fig. 5.

RIPK1 inhibition by Nec-1 confers hepatocytes resistance to oxidative stress, isolated hepatocytes were cultured with different Nec-1 concentrations or the vehicle alone for 1 h and then treated with 250 μM H₂O₂ or 2 mM SNAP. (A and D) LDH was measured 6 h after H₂O₂ (A) or SNAP (D) administration (n = 6). (B and E) Cell viability was measured 6 h after H₂O₂ (B) or SNAP (E) administration using WST-8 (n = 6). (C and F) The cells were stained with Hoechst 33342 and propidium iodide 6 h after adding H₂O₂ (C) or SNAP (F), and then examined by fluorescent microscopy. (G and H) The antioxidant effect of Nec-1 was evaluated using the ABTS free-radical decolorization assay. An Nec-1 inactive control and l(+)-ascorbic acid were used as controls. Samples were dissolved in either distilled water (G) or ethanol (H) (n = 6). ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001.