Table 3. Intracellular yeast fate analysis identifies yeast with tubular mitochondria as resistant and non-proliferative cells.

	Mitochondrial morphology				Fisher’s exact test
	Tubular		Non-tubular
	Count	%	Count	%
AIg54
Total	69	24.0	219	76.0
Killed	0	0	49	22.4	P<0.0001
Proliferating	1	1.4	35	16.0	P=0.0006
Quiescent	68	98.6	134	61.2	P<0.0001
AIg56
Total	17	9.9	154	90.1
Killed	0	0	2	1.3	P=1.0
Proliferating	10	58.8	80	51.9	P=0.62
Quiescent	7	41.2	74	48.1	P=0.62

The fate of individual intracellular yeasts and their respective mitochondrial phenotype was scored from time-lapse images over 12 h (730 min). Fewer intracellular yeast were killed when tubular mitochondrial morphology has developed (Fisher’s exact test, P<0.0001). Intracellular yeast proliferation was more often observed in yeasts from the outbreak strain AIg54 not presenting with tubular mitochondria (Fisher’s exact test, P=0.0006). Intracellular AIg54 cells with tubular mitochondrial morphology were more often quiescent than intracellular AIg56 cells with tubular mitochondria (Fisher’s exact test, P<0.0001). Data were obtained from the outbreak strain AIg54 and non-outbreak strain AIg56 with HEM15-GFP-tagged mitochondria in four independent experimental repeats examining 288 and 173 yeasts, respectively, and data analysed using Fisher’s exact test.