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Scheme of the AMIS procedure for fluoroquinolones. Firstly, ciprofloxacin standards, Ab171-MB suspension and 11-HRP solution were mixed and incubated for 30 min with gentle stirring. After three washing steps using PBST buffer, performed with the aid of a magnetic rack, the modified magnetic beads are captured by the m-GEC. Then, the electrode is transported to the electrochemical cell using a three-electrode setup for the amperometric measurement.
