Figure 2.

Representative nuclear stained images (a) and resulting trajectory information (b–d) obtained from the ACTIVE tracking approach. Cells were stained with Hoechst 33342 nuclear dye after seeding on wrinkled, non-wrinkled or TCPS substrates. (a) Representative images for wrinkled substrates of the three seeding densities (5000, 10 000 and 20 000 cells cm⁻²) used in the anisotropy study illustrate the very low contrast used in this work. (b–d) The resulting trajectory information obtained from the ACTIVE analysis qualitatively shows that wrinkled samples yield directional migration of C3H10T1/2 mouse fibroblast cells. Representative tracks for non-wrinkled and TCPS isotropic substrates qualitatively show no preferential directional migration. For the wrinkled images, black double headed arrows indicate wrinkle direction.