Figure 4.

Representative velocity-autocorrelation analyses obtained from the ACTIVE tracking approach. Decomposition of x- and y-velocity (I and II, respectively) autocorrelation data reveals a distinct preferential migration of cells on wrinkled topographies corresponding to the grooved direction. The non-wrinkled and TCPS control substrates demonstrated x- and y-velocity data with similar exponential fitting for both components. Time constants for wrinkled exponential fits demonstrated statistically significant differences between x- and y-velocity component data. W, wrinkled; N, non-wrinkled; T, TCPS; density = 5000 cells cm⁻²; n = 4. (Online version in colour.)