Table 1.
P. aeruginosa strains used for this study.
| Strains and plasmids | Details | Study |
|---|---|---|
| PLASMIDS | ||
| pMEXGFP | pMEX9 derived promoter-probe vector; egfp Gmr | Toyofuku et al., 2013 |
| pMEXdsREDex | pMEX9 derived promoter-probe vector; dsredexpress Gmr | This study |
| pMNarK1-GFP | narK1 promoter region fused to egfp in pMEXGFP | This study |
| pMNirS-dsREDex | nirS promoter region fused to dsredexpress in pMEXdsREDex | This study |
| pMNorC-GFP | norC promoter region fused to egfp in PMEXGFP | This study |
| pMNosR-dsREDex | nosR promter region fused to dsredexpress in pMEXdsREDex | This study |
| pET15b | Expression vector Apr | Novagen, Madison, WI, USA |
| pG19nir | nirS to nirN deletion cassette in PG19II | Toyofuku et al., 2013 |
| pG19nos | nosZ deletion cassette in PG19II | This study |
| STRAINS | ||
| E. coli | ||
| S17-1 | Mobilizer strain | Simon et al., 1986 |
| P. aeruginosa | ||
| PAO1 MH340 | WT | Pseudomonas Genetic Stock Center |
| PAO1 egfp | Background control | Toyofuku et al., 2013 |
| PAO1 dsredexpress | Background control | This study |
| PAO1 narK1-eGFP | Nitrate reductase reporter | This study |
| PAO1 nirS-dsredexpress | Nitrite reductase reporter | This study |
| PAO1 norC-eGFP | Nitric oxide reductase reporter | This study |
| PAO1 nosR-dsredexpress | Nitrous oxide reductase reporter | This study |
| Holloway PAO1 | WT | Holloway et al., 1979 |
| Holloway ΔnirS-N | nirS to nirN deleted mutant | Toyofuku et al., 2013 |
| Holloway ΔnosZ | nosZ deleted mutant | This study |
| I M/NM | Clinical isolate | Kolpen et al., 2014c |
| II M/NM | Clinical isolate | Kolpen et al., 2014c |
| III M/NM | Clinical isolate | Kolpen et al., 2014c |
| IV M/NM | Clinical isolate | Kolpen et al., 2014c |
| V M/NM | Clinical isolate | Kolpen et al., 2014c |
| VI NM | Clinical isolate | Kolpen et al., 2014c |
| VII NM | Clinical isolate | Kolpen et al., 2014c |
Gmr (Gentamicin resistant), Apr (Ampicillin resistant), Mucoid (M), Non-mucoid (NM), Wild type (WT).