Table 2.

Primer sequences used in this study.

Primers	Sequence	Study
REPORTER PLASMIDS
Gfp F1	5′ – ACGCCAAGCTTGCATGCCTG – 3′	Toyofuku et al., 2013
pET15b-kpn	5′ – GGGGTACCACTTTAAGAAGGAGATATACCATGG – 3′	Toyofuku et al., 2013
dsred R1	5′ – GGGCTCGAGCTACAGGAACAGGTGGTGGCGGC – 3′	This study
dsred R2	5′ – CGAGCTCCTACAGGAACAGGTGGTGGCGGC – 3′	This study
pNarK1 F	5′ – GGAATTCCCGGCGTGGTTGATCACCGC – 3′	This study
pNarK1 R	5′ – TTCAAGCTTAGGCCAGGCCGTAGATCGGG – 3′	This study
pNirS F	5′ – CCCAAGCTTCTTTCATGTCGTCCTTGGCGTG – 3′	This study
pNirS R	5′ – CCCAAGCTTGCCCTTGAGCAATACCGGCAG – 3′	This study
pNorC F	5′ – CGGAATTCGCGAGCGCTTCATGGAGCTGG – 3′	This study
pNorC R	5′ – GCCAAGCTTGCCGAAATAGATGTTCCTGGCC – 3′	This study
pNosR F	5′ – CGGGATCCCCTGGTACCGTTACCTGAAGGC – 3′	This study
pNosR R	5′ – CCCAAGCTTGGATCACCTGCAGGTTGATCGG – 3′	This study
MUTANTS
ΔnirS-N F1	5′ – CGGAATTCCCAGTAGAGGGTGAAGAAAGTGTCGTGC – 3′	Toyofuku et al., 2013
ΔnirS-N R1	5′ – CGGGATCCGACCGGAATCAAGATTGCGTTGCTTTGC – 3′	Toyofuku et al., 2013
ΔnirS-N F2	5′ – CGGGATCCGGTCGAGACGCCATCAACGGATATCC – 3′	Toyofuku et al., 2013
ΔnirS-N R2	5′ – CCGAAGCTTGCGATGTTCACCATGATGAACTACGAGC – 3′	Toyofuku et al., 2013
ΔnosZ F1	5′ – CGCAAGCTTGGGTCTGGTACCAGAAGAGTTTCCAGG – 3′	This study
ΔnosZ R1	5′ – GACTAGTGTCGCTCATCTCGTGTCGTCCTCAAGGG – 3′	This study
ΔnosZ F2	5′ – GACTAGTCCGCAACAGACCTCGTCGATCACC – 3′	This study
ΔnosZ R2	5′ – CGCAAGCTTCGAGTAGGTGATGTAGTTCATCAGGATGCC – 3′	This study
qPCR
narK1F	5′ – CTCCTCCTGCTTCATGTTGCT – 3′	This study
narK1R	5′ – TGCTGCTTGATCGCGTAGTG – 3′	This study
nirS F	5′ – GGCGGCTCGCTGTTCAT – 3′	This study
nirS R	5′ – TGAAGGTGGTGTCGACGTAGAG – 3′	This study
norC F	5′ – AAGGCATGGCCAGGAACAT – 3′	This study
norC R	5′ – CCAGGAACAGCAGGATGAAGA – 3′	This study
nosZ F	5′ – CGCTGCACACCACTTTCG – 3′	This study
nosZ R	5′ – CACCAACTGGCTGTCGATGA – 3′	This study
rpoD F	5′ – ACAAGATCCGCAAGGTACTGAAG –3′	This study
rpoD R	5′ – CGCCCAGGTGCGAATC – 3′	This study

Forward (F), Reverse (R).