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. 2014 Aug 20;25(5):277–287. doi: 10.1089/hgtb.2014.004

FIG. 3.

FIG. 3.

TCR expression and phenotype of T-cells in the GMP-grade cell products. (A) TCR expression was determined on the final day of culturing by flow cytometric analysis using anti-CD3, anti-CD8 antibody, and HLA-A*02:01 MART-1(26–35 A>L) tetramers. Cells are gated on CD3+ cells. The number in the upper-right corner indicates the percentage of tetramer+ cells of CD8+ cells. The number in the lower-right corner indicates the percentage of tetramer+ cells of CD8− cells. In the validation run indicated with “*,” only a single cycle of retroviral transduction was performed. (B) Cell products obtained in the three independent production runs were analyzed simultaneously after thawing and overnight recovery. All plots show cells gated on tetramer+ CD8+ lymphocytes. Cells were stained for CD27 and CD28 expression (top row), CD62L and CD45RA expression (middle row), or CD45RA and CD45RO expression (bottom row).