FIG. 4.

Scheme of the p11Mut and the different PPRHs designs. (A) Scheme representing the p11Mut plasmid containing the nonsense mutation in the dhfr minigene. (B) The dhfr minigene contained the six exons of the hamster dhfr gene and its intron 1. The point mutation is localized in exon 2, where the repair-PPRHs are targeted. (C) Five repair-PPRHs were designed against the exon 2 of the dhfr gene. Two of them (HpE2rep and HpE2link) contained only a single extended sequence of DNA bearing the repaired nucleotide; HpE2link-ds/RO18 was complemented with an extra oligonucleotide hybridized to the extension of the repair-PPRH; dHpE2link-rep carried an oligonucleotide covalently bound to the extended tail by a loop, and the last repair-PPRH (HpE2ext/RO24) contained an extension tail until 2nt before the point mutation, and carried an extra oligonucleotide that did contain the repaired nucleotide. Color images available online at www.liebertpub.com/hgtb