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. 2014 Sep 15;25(5):288–302. doi: 10.1089/hgtb.2014.049

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Copyright 2014, Mary Ann Liebert, Inc.

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FIG. 5. — Repair-PPRHs binding to their target sequence. (A) Binding between the radiolabeled dsDNA duplex (Dup50) and the different repair-PPRHs. Lane 1, labeled ³²P-polypurine/³²P-polypyrimidine dsDNA (Dup50) (0.67 nM); lane 2, ³²P-polypyrimidine ssDNA (pPy50) (0.27 nM); lane 3, ³²P-polypurine ssDNA (pPu50) (0.42 nM); lane 4, ³²P-Dup50 plus HpE2rep; lane 5, ³²P-pPy50 plus HpE2rep; lane 6, ³²P-Dup50 plus HpE2link; lane 7, ³²P-pPy50 plus HpE2link; lane 8, ³²P-Dup50 plus HpE2link-ds; lane 9, ³²P-pPy50 plus HpE2link-ds; lane 10, ³²P-Dup50 plus HpE2ext; lane 11, ³²P-pPy50 plus HpE2ext; lane 12, ³²P-Dup50 plus dHpE2link-rep; lane 13, ³²P-pPy50 plus dHpE2link-rep; lane 14, ³²P-Dup50 plus RO18; lane 15, ³²P-Dup50 plus RO24. (B) Binding of a longer dsDNA duplex (Dup575) and the radiolabeled HpE2rep. Lane 1, labeled ³²P-HpE2rep (0.35 nM); lane 2, ³²P-HpE2rep plus Dup576. (+) indicate unlabelled oligonucleotides present in the reaction. Color images available online at www.liebertpub.com/hgtb