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. 2014 Sep 15;25(5):288–302. doi: 10.1089/hgtb.2014.049

FIG. 6.

FIG. 6.

Levels of DHFR mRNA and protein in repaired cells. (A) DHFR mRNA in repaired DG44 cells. RNA was extracted from repaired colonies of DG44 cells upon transfection with p11Mut and PPRHs. DHFR mRNA levels were determined using qRT-PCR and referred to the levels of endogenous APRT control. DHFR mRNA levels of the repaired colonies were referred to the mRNA levels of the positive control p11 (DG44-p11). (B) DHFR protein levels in repaired DG44 cells. Total protein extracts were obtained from the repaired DG44 colonies upon transfection with p11Mut and PPRHs and analyzed by Western blot. Protein levels were normalized using tubulin. Protein levels of the repaired colonies were referred to the protein levels of the positive control p11 (DG44-p11). (C) DHFR mRNA levels in repaired DG44-p11Mut cells. RNA was extracted from surviving DG44-p11Mut cell colonies upon transfection with PPRHs. DHFR mRNA levels were determined using qRT-PCR and referred to the levels of endogenous APRT control. DHFR mRNA levels of the repaired colonies were referred to the mRNA levels of the positive control p11 (DG44-p11). (D) DHFR protein levels in repaired DG44-p11Mut cells. Total protein extracts were obtained from DG44-p11Mut upon transfection with PPRHs and analyzed by Western blot. Protein levels were normalized using tubulin. Protein levels of the repaired colonies were referred to the protein levels of the positive control p11 (DG44-p11).