FIG. 9.

DNA sequencing and DHFR protein of repaired cell colonies. (A) Sequences of the nonsense mutant of the dhfr minigene p11Mut, and those of repaired cells using different repair-PPRHs upon transfection in either DG44 or DG44p11Mut cell lines. (B) Repaired cell colony upon transfection of DG44 cells with the mutated version of the dhfr minigene (p11Mut) and HpE2rep, and selection in selective medium (w/o hypoxanthine). (C) Representative image of a Western blot showing the DHFR protein levels of DG44-p11 (positive control), DG44-p11Mut (negative control), and three repaired colonies obtained from DG44 upon transfection with p11Mut and HpE2rep, and three colonies from DG44-p11Mut transfected with HpE2rep. Color images available online at www.liebertpub.com/hgtb