Table 1.
Sequence of PPRHs and Oligonucleotides Used in the Study
| Name | Sequence (5′ to 3′) Sequences used in vitro |
|---|---|
| Hp13 | GGAAAAAGGAGGA-(T)5-AGGAGGAAAAAGG |
| pPy13 | CCTTTTTCCTCCT |
| pPu13 | AGGAGGAAAAAGG |
| pPy25 | TTGATGCCTTTTTCCTCCTGGACTT |
| pPu25 | AAGTCCAGGAGGAAAAAGGCATCAA |
| RO10 | TTTTTCCTCC |
| pPy61 | TCTTTCTGTTAGCCTTTCTTCTCATAGACTTAAAATTTAT |
| ACTTGATGCCTTTTTCCTCCT | |
| pPu61 | AGGAGGAAAAAGGCATCAAGTATAAATTTTAAGTCTATGA GAAGAAAGGCTAACAGAAAGA |
| Hp23 | AGAAAGAAAAAAGGAAAGAAGAG-(T)5-GAGAAGAAAGGAAAAAAGAAAGA |
| RO20 | TAGACTTCAAATTTATACTT |
| Sequences used in in vitro and in cellular experiments | |
|---|---|
| HpE2rep | GGAGGAGGAGGAG-(T)5-GAGGAGGAGGAGGTCATTCTTTGGAAGTACTTGAATTC |
| HpE2link | GAATTCAAGTACTTCCAAAGTTTTTGGAGGAGGAGGAG-(T)5-GAGGAGGAGGAGG |
| HpE2link-ds | GAATTCAAGTACTTCCAATTTTTTTGGAGGAGGAGGAG-(T)5-GAGGAGGAGGAGG |
| RO18 | TTGGAAGTACTTGAATTC |
| HpE2ext | CCAAAGAATGTGGAGGAGGAGGAG-(T)5-GAGGAGGAGGAGG |
| RO24 | CATTCTTTGGAAGTACTTGAATTC |
| dHpE2link-rep | CTTTGGAAGTACTTGAATTCTTTTTGAATTCAAGTACTTCCAAAGTTTTTGGAGGAGGAGGAG-(T)5-GAGGAGGAGGAGG |
| p11Mut-pPy50 | GAACGAATTCAAGTAGTTCCAAAGAATGACCACCACCTCCTCAGTGGAAG |
| p11Mut-pPu50 | CTTCCACTGAGGAGGTGGTGGTCATTCTTTGGAACTACTTGAATTCGTTC |
| HpE2rep-NH | AAGAAGAAGAAGA-(T)5-GAGGAGGAGGAGGTCATTCTTTGGAAGTACTTGAATTC |
| HpE2rep-WC | CCTCCTCCTCCTC-(T)5-GAGGAGGAGGAGGTCATTCTTTGGAAGTACTTGAATTC |
| HpE6rep | CATCAAGTATAAATTTGAAGTCTATGAGAAGAAAGGCTAACAGAAAGA-(T)5-AGAAAGACAATCGGAAAGAAGAG |
PPRH, polypurine reverse Hoogsteen hairpins; ODNs, oligodeoxynucleotides.
The names and sequences of the PPRHs and ODNs are indicated. Mismatches in the PPRHs compared with their targets are underlined. The nonsense mutation is in bold, and the corresponding corrected nucleotide in the repair-PPRHs is in italic.