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. 2014 Jun 11;2(6):e12039. doi: 10.14814/phy2.12039

Figure 4.

Figure 4.

Loss of α(E)‐catenin reduces wound healing. Repair of a scratch in monolayers of C2 cells was inhibited as compared to NT3 cells in serum‐free (SF) conditions. (A) Representative images of NT3 and C2 cells 24 h following injury in serum‐free conditions; the inset in each image is the T0 time point. (B) Quantitative assessment of wound healing at 24 h is shown in both serum and serum‐free conditions; *indicates a statistically significant difference between C2 and NT3, or C2 SF and NT3 SF (n = 6). (C) Gene expression of E‐cadherin, N‐cadherin and N‐CAM in control and migrating NT3 and C2 cells. A similar pattern is seen for E‐cadherin and N‐CAM in migrating NT3 and C2 cells, that is, a decrease in E‐cadherin and increase in N‐CAM in both cells. However, migrating NT3 cells exhibit an increase in N‐cadherin gene expression that is not seen in C2 cells. (D) Inhibition of N‐cadherin via a blocking antibody (GC‐4) inhibits repair at 24 h in 5% FBS or serum‐free media.