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. 2014 Jun 11;2(6):e12018. doi: 10.14814/phy2.12018

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© 2014 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Determining the presence of lysoPC in egg lecithin by TLC. (A) 0.5 mg of lysoPC (left) and 2 mg egg lecithin (right) in chloroform were separated on silica gel 60 plates using chloroform:methanol:acetic acid:water (50:37.5:3.5:2) (v:v:v:v) as the solvent system. The plate was stained by choline staining followed by 20% sulfuric acid staining with 100°C heating until the color developed. (B) 0.5 mg PC (left), 5 mg lysoPC (middle), 0.5 mg lecithin (right) in chloroform:methanol:water (12:7:1) (v:v:v) were separated on silica gel 60 plates using chloroform:ethanol:water:triethylamine (30:35:7:3.5) (v:v:v:v) as the solvent system. The plate was stained by PL staining with 100°C heating until the color developed.