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. 2014 Sep 23;77(10):2241–2247. doi: 10.1021/np500498j

Figure 1.

Figure 1

Human neutrophil 22-OH-PD1 (6) matches synthetic material. Representative MRM chromatograms. n = 3. Selected ion chromatogram (m/z 375–153) depicting (A) 22-OH-PD1 (6) obtained from human peripheral blood neutrophils (5 × 107/mL) incubated with 0.1 mg of opsonized zymosan and PD1 (3) (10 ng/mL, 37 °C, 30 min, DPBS+/+, pH = 7.45). n = 3 human neutrophil preparations. (B) Synthetic material (inset: characteristic UV-absorption spectrum in MeOH, ±1 nm). (C) Co-injection of human neutrophil 22-OH-PD1 (6) with synthetic 6.