Figure 4.

PD1 (3) and 22-OH-PD1 (6) inhibit human PMN chemotaxis and adhesion. Purified human PMN were pretreated with 22-OH-PD1 (6), PD1 (3), or vehicle control (15 min, 37 °C). PMN were then cultured on a ChemoTx chamber (90 min, 37 °C in 5% CO₂), and PMN chemotaxis toward (A) LTB₄ (10 nM) or (B) IL-8 (100 ng/mL) was measured. Cell chemotaxis was quantified, and samples were normalized to vehicle control. Results show four independent experiments, presented as the mean ± SEM. (C) PMN were pretreated with 22-OH-PD1 (6), PD1 (3), or vehicle control (10 min, RT) and stimulated with 10 nM LTB₄ (10 min, RT). PMN were then passed through a flow chamber at 0.5 mL/min for 5 min. Six random fields of view were recorded, and the number of adhered PMN was quantified. Representative images of adhered PMN (left panel) and quantification (right panel) are shown. Results were obtained from three independent experiments, shown as the mean ± SEM. All results were compared to vehicle control using a paired t test; statistical significance defined as *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.