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. Author manuscript; available in PMC: 2014 Oct 24.
Published in final edited form as: Chem Res Toxicol. 2009 Mar 16;22(3):433–445. doi: 10.1021/tx8002752

Figure 3.

Figure 3

ELISA validation of microarray results. Equivalent numbers of cells (4 × 106/well) were stimulated with LPS (1 µg/mL) in the absence or presence of 35 µM HNE for 24 h. CSF3 (colony stimulating factor 3 (granulocyte)) and MMP-9 (matrix metalloproteinase 9) released into the culture medium were analyzed by ELISA. Fold changes (HNE-treated stimulated cells relative to stimulated controls) are shown (X̄ ± SD for triplicate measurements, representative of three independent experiments).