Table 3.
Select Gene Expression Changes Mediated by HNEa
| Gene Symbol |
Description | Fold Change | MGI Gene ID |
||
|---|---|---|---|---|---|
| 6 h | 24 h | ||||
| Cell Cycle | Atm | ataxia telangiectasia mutated homolog (human) | 1.9 | 107202 | |
| Atr | Ataxia telangiectasia and rad3 related | 3.8 | 108028 | ||
| Bub1 | budding uninhibited by benzimidazoles 1 homolog (S. cere |
−2.7 | 1100510 | ||
| Bub1b | budding uninhibited by benzimidazoles 1 homolog, beta (S. |
−2.8 | 1333889 | ||
| Ccna2 | cyclin A2 | −5.1 | 108069 | ||
| Ccnb1 | cyclin B1 | −5.2 | 88302 | ||
| Ccnd1 | cyclin D1 | 1.9 | 88313 | ||
| Ccnf | cyclin F | −4.1 | 102551 | ||
| Ccng2 | cyclin G2 | −2.9 | 1095734 | ||
| Ccr1 | chemokine (C-C motif) receptor 1 | −3.1 | 104618 | ||
| Cdc20 | cell division cycle 20 homolog (S. cerevisiae) | −2.3 | 1859866 | ||
| Cdc25a | cell division cycle 25 homolog A (S. pombe) | −1.9 | 103198 | ||
| Cdc25c | cell division cycle 25 homolog C (S. pombe) | −3.5 | 88350 | ||
| Cdk6 | cyclin-dependent kinase 6 | 1.9 | 1277162 | ||
| Chek1 | checkpoint kinase 1 homolog (S. pombe) | −2.3 | 1202065 | ||
| Dbf4 | DBF4 homolog (S. cerevisiae) | −2.0 | 1351328 | ||
| E2f2 | E2F transcription factor 2 | 2.0 | 1096341 | ||
| Fen1 | flap structure specific endonuclease 1 | 2.5 | 102779 | ||
| Gadd45a | growth arrest and DNA-damage-inducible 45 alpha | 2.5 | 107799 | ||
| Mki67 | antigen identified by monoclonal antibody Ki 67 | −4.0 | 106035 | ||
| Msh5 | mutS homolog 5 (E. coli) | 6.2 | 1329021 | ||
| Mutyh | mutY homolog (E. coli) | 3.1 | 1917853 | ||
| Mxd1 | MAX dimerization protein 1 | −4.7 | 96908 | ||
| Ndc80 | NDC80 homolog, kinetochore complex component (S. cerevisia |
−2.4 | 1914302 | ||
| Pa2g4 | proliferation-associated 2G4 | 2.3 | 894684 | ||
| Pcna | proliferating cell nuclear antigen | 2.3 | 97503 | ||
| Plk1 | polo-like kinase 1 (Drosophila) | −8.6 | 97621 | ||
| Rad23a | RAD23a homolog (S. cerevisiae) | 2.2 | 105126 | ||
| Rad51 | RAD51 homolog (S. cerevisiae) | 16.3 | 97890 | ||
| Rbl1 | retinoblastoma-like 1 (p107) | 2.7 | 103300 | ||
| Riok3 | RIO kinase 3 | 1.9 | 1914128 | ||
| Sass6 | spindle assembly 6 homolog (C. elegans) | −2.4 | −4.6 | 1920026 | |
| Suv39h1 | suppressor of variegation 3–9 homolog 1 (Drosophila) |
2.4 | 1099440 | ||
| Tgfb | transforming growth factor, beta 1 | 2.0 | 98725 | ||
| Xaf1 | XIAP associated factor 1 | −2.1 | −87.4 | 3772572 | |
| Zwilch | Zwilch, kinetochore associated, homolog (Drosophila) |
−2.3 | 1915264 | ||
| Cell Signaling | Fcgr1a | Fc fragment of IgG, high affinity Ia, receptor (CD64) | −4.4 | −13.6 | 95498 |
| Fcgr2b | Fc fragment of IgG, low affinity IIb, receptor (CD32) | −7.7 | −2.9 | 95499 | |
| Cellular Development | Cd83 | CD83 molecule | −2.4 | −2.4 | 1328316 |
| Ifi16 | interferon, gamma-inducible protein 16 | −4.1 | −11.6 | 96429 | |
| Irf7 | interferon regulatory factor 7 | −3.4 | −51.3 | 1859212 | |
| Mafb | v-maf musculoaponeurotic fibrosarcoma oncogene homolog B (avian) |
−4.4 | −2.2 | 104555 | |
| Dyserythropoiesis | Ccl5 | chemokine (C-C motif) ligand 5 | −22.5 | 98262 | |
| Traf3 | Tnf receptor-associated factor 3 | −2.3 | 108041 | ||
| Tsc22d3 | TSC22 domain family, member 3 | −3.6 | 1196284 | ||
| ECM degradation | Mmp9 | matrix metalloproteinase 9 | −4.0 | 5.3 | 97011 |
| Timp1 | tissue inhibitor of metalloproteinase 1 | −8.5 | - | 98752 | |
| Gene Expression | Axud1 | AXIN1 up-regulated 1 | −3.0 | −1.8 | 2387989 |
| Batf2 | basic leucine zipper transcription factor, ATF-like 2 | −1.8 | −3.1 | 1921731 | |
| Ddx58 | DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 | −2.4 | −14.2 | 2442858 | |
| Mx1 | myxovirus (influenza virus) resistance 1, interferon- inducible protein p78 (mouse) |
−7.9 | −43.4 | 97243 | |
| Pcgf5 | polycomb group ring finger 5 | −1.8 | −2.1 | 1923505 | |
| Phf11 | PHD finger protein 11 | −2.9 | −13.7 | 1918441 | |
| Sp100 | SP100 nuclear antigen | −2.8 | −4.4 | 109561 | |
| Glutathione Metabolism | G6pd2 | glucose-6-phosphate dehydrogenase 2 | 1.8 | 2.0 | 105977 |
| G6pdx | glucose-6-phosphate dehydrogenase X-linked | 2.1 | 105979 | ||
| Gclc | glutamate-cysteine ligase, catalytic subunit | 8.6 | 104990 | ||
| Gclm | glutamate-cysteine ligase , modifier subunit | 7.6 | 104995 | ||
| Gss | glutathione synthetase | 2.4 | 95852 | ||
| Gsta1 | glutathione S-transferase, alpha 1 (Ya) | 7.2 | 30.7 | 1095417 | |
| Gstp1 | glutathione S-transferase, pi 1 | 1.8 | 95865 | ||
| Idh1 | isocitrate dehydrogenase 1 (NADP+), soluble | 3.3 | 96413 | ||
| Pgd | phosphogluconate dehydrogenase | 2.4 | 97553 | ||
| Immune Response | Adrb2 | adrenergic, beta-2-, receptor, surface | 4.1 | 2.2 | 87938 |
| C5r1 | complement component 5, receptor 1 | 3.5 | 88232 | ||
| Casp4 | caspase 4, apoptosis-related cysteine peptidase | −2.2 | −2.4 | 107700 | |
| Ccl17 | chemokine (C-C motif) ligand 17 | 3.1 | 1329039 | ||
| Ccl2 | chemokine (C-C motif) ligand 2 | −44.8 | 98259 | ||
| Ccl22 | chemokine (C-C motif) ligand 22 | −12.3 | 1306779 | ||
| Ccl4 | chemokine (C-C motif) ligand 4 | −2.1 | 98261 | ||
| Ccl6 | chemokine (C-C motif) ligand 6 | −3.1 | 98263 | ||
| Ccl7 | chemokine (C-C motif) ligand 7 | −22.6 | −2.3 | 99512 | |
| Ccr1 | chemokine (C-C motif) receptor 1 | −3.1 | 104618 | ||
| Cd14 | CD14 antigen | 2.2 | 88318 | ||
| Cd300lf | CD300 antigen like family member F | −3.9 | 2442359 | ||
| Cd40 | CD40 antigen | −4.3 | 88336 | ||
| Cd44 | CD44 antigen | −2.6 | 88338 | ||
| Cd86 | CD86 antigen | −1.8 | 101773 | ||
| Cenpa | centromere protein A | −2.3 | 88375 | ||
| Cfb | complement factor B | −7.4 | 105975 | ||
| Clec12a | C-type lectin domain family 12, member a | −1.9 | 3040968 | ||
| Clec2d | C-type lectin domain family 2, member d | −4.1 | 2135589 | ||
| Clec4n | C-type lectin domain family 4, member n | −2.4 | 1861231 | ||
| Clec5a | C-type lectin domain family 5, member a | −1.8 | 1345151 | ||
| Csf2 | colony stimulating factor 2 (granulocyte- macrophage) |
−12.1 | 1339752 | ||
| Csf3 | colony stimulating factor 3 (granulocyte) | −18.2 | 18.0 | 1339751 | |
| Cxcl1 | chemokine (C-X-C motif) ligand 1 | 2.7 | 108068 | ||
| Cxcl14 | chemokine (C-X-C motif) ligand 14 | −2.7 | 1888514 | ||
| Cxcr4 | chemokine (C-X-C motif) receptor 4 | 2.5 | 109563 | ||
| Dcn | decorin | −4.0 | 94872 | ||
| Ercc1 | excision repair cross-complementing rodent repair deficient |
−1.8 | 95412 | ||
| F10 | coagulation factor X | 4.1 | 103107 | ||
| Fbxo5 | F-box protein 5 | −4.0 | 1914391 | ||
| Gbp1 | guanylate binding protein 1 | −3.1 | −16.8 | 95666 | |
| Gbp3 | guanylate nucleotide binding protein 3 | −3.7 | 1926263 | ||
| Gbp5 | guanylate nucleotide binding protein 5 | −5.9 | 2429943 | ||
| H28 | histocompatibility 28 | −6.3 | 95975 | ||
| Hdc | histidine decarboxylase | −8.8 | −3.1 | 96062 | |
| Icam1 | intercellular adhesion molecule 1 | 1.8 | 7.5 | 96392 | |
| Igf1 | insulin-like growth factor 1 | 2.1 | 96432 | ||
| Il10 | interleukin 10 | −4.7 | 96537 | ||
| Il10ra | interleukin 10 receptor, alpha | 3.7 | 96538 | ||
| Il13ra1 | interleukin 13 receptor, alpha 1 | −3.4 | 105052 | ||
| Il18 | interleukin 18 | −2.0 | 107936 | ||
| Il18rap | interleukin 18 receptor accessory protein | −2.8 | 1338888 | ||
| Il1a | interleukin 1 alpha | −66.6 | 96542 | ||
| Il1b | interleukin 1 beta | −32.7 | 96543 | ||
| Il1f6 | interleukin 1 family, member 6 | −12.6 | 1859324 | ||
| Il1rl1 | interleukin 1 receptor-like 1 | −3.6 | 98427 | ||
| Il1rn | interleukin 1 receptor antagonist | −6.8 | 96547 | ||
| Il27 | interleukin 27 | −6.4 | 2384409 | ||
| Il4ra | interleukin 4 receptor, alpha | −2.9 | 105367 | ||
| Il6 | interleukin 6 | −43.3 | −11.2 | 96559 | |
| Isg20 | interferon stimulated exonuclease gene 20kDa | −3.1 | −7.6 | 1928895 | |
| Ltb | lymphotoxin B | −1.9 | 104796 | ||
| Nlrc4 | NLR family, CARD domain containing 4 | 2.3 | 2.7 | 3036243 | |
| Oasl2 | 2'–5' oligoadenylate synthetase-like 2 | −5.1 | 1344390 | ||
| Pgdfb | platelet derived growth factor, B polypeptide | 3.2 | 97528 | ||
| Pla2g7 | phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) |
3.1 | 10.7 | 1351327 | |
| Pou2f2 | POU domain, class 2, transcription factor 2 | −1.9 | 101897 | ||
| Rsad2 | radical S-adenosyl methionine domain containing 2 | −4.4 | 1929628 | ||
| Tnf | tumor necrosis factor | 8.7 | 104798 | ||
| Traf3ip2 | Traf3 interacting protein 2 | −1.8 | 2143599 | ||
| Ube2L6 | ubiquitin-conjugating enzyme E2L 6 | −1.8 | −2.3 | 1914500 | |
| Vegfa | vascular endothelial growth factor A | −2.4 | 103178 | ||
| Interferon-associated Signaling or Regulation |
H2-Bf | histocompatibility 2, complement component factor B |
−51.0 | 105975 | |
| H2-DMb2 | histocompatibility 2, class II, locus Mb2 | −5.1 | 95923 | ||
| H2-Q1 | histocompatibility 2, Q region locus 1 | −3.2 | 95928 | ||
| H2-Q5 | histocompatibility 2, Q region locus 5 | −2.1 | 95934 | ||
| H2-T23 | histocompatibility 2, T region locus 23 | −9.4 | 95957 | ||
| H2- T9/H2- T22 |
histocompatibility 2, T region locus 9;histocompatibility 2, T region locus 22 |
−5.2 | 95965 | ||
| Ifi202b | interferon activated gene 202B | −3.8 | 1347083 | ||
| Ifi203 | interferon activated gene 203 | −3.7 | 96428 | ||
| Ifi204 | interferon activated gene 204 | −3.7 | 96429 | ||
| Ifi205 | interferon activated gene 205 | −4.3 | 101847 | ||
| Ifi47 | interferon gamma inducible protein 47 | −3.0 | 99448 | ||
| Ifih1 | interferon induced with helicase C domain 1 | −2.0 | 1918836 | ||
| Ifit1 | interferon-induced protein with tetratricopeptide repeats 1 |
−3.6 | 99450 | ||
| Ifit2 | interferon-induced protein with tetratricopeptide repeats 2 |
−6.3 | 99449 | ||
| Ifit3 | interferon-induced protein with tetratricopeptide repeats 3 |
−3.3 | 1101055 | ||
| Irf8 | interferon regulatory factor 8 | −2.6 | 96395 | ||
| Jak2 | Janus kinase 2 | −1.7 | 96629 | ||
| Mx2 | myxovirus (influenza virus) resistance 2 | −7.3 | 97244 | ||
| Oas1a | 2’–5’ oligoadenylate synthetase 1A | −2.0 | 2180860 | ||
| Ptges | prostaglandin E synthase | 4.7 | 1927593 | ||
| Stat1 | signal transducer and activator of transcription 1 | −2.2 | −10.8 | 103063 | |
| Stat3 | signal transducer and activator of transcription 3 | −2.1 | 103038 | ||
| Stat5a | signal transducer and activator of transcription 5A | −2.1 | 103036 | ||
| Metabolic Process | Adh7 | alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide |
8.0 | 2.3 | 87926 |
| Hbp1 | high mobility group box transcription factor 1 | 2.9 | 894659 | ||
| Mov10 | Mov10, Moloney leukemia virus 10, homolog (mouse) |
−2.2 | −2.5 | 97054 | |
| Oas3 | 2'–5'-oligoadenylate synthetase 3, 100kDa | −2.4 | −8.3 | 2180850 | |
| Parp12 | poly (ADP-ribose) polymerase family, member 12 | −2.1 | −4.9 | 2143990 | |
| Serpinb1b | serine (or cysteine) peptidase inhibitor, clade B, member 1b |
2.8 | 4.3 | 2445361 | |
| Tiparp | TCDD-inducible poly(ADP-ribose) polymerase | −1.8 | −2.9 | 2159210 | |
| NF-kB signaling | Ikbke | inhibitor of kappaB kinase epsilon | 3.2 | 1929612 | |
| Nfkbia | nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, alpha |
1.8 | 104741 | ||
| Nfkbie | nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon |
3.6 | 1194908 | ||
| Oxidative Stress Response |
Abcc1 | ATP-binding cassette, sub-family C (CFTR | 2.1 | 102676 | |
| Akr1a4 | aldo-keto reductase family 1, member A4 (aldehyde reducta |
1.8 | 1929955 | ||
| Aox1 | aldehyde oxidase 1 | 2.2 | 88035 | ||
| Cat | catalase | 3.1 | 88271 | ||
| Dnajb4 | DnaJ (Hsp40) homolog, subfamily B, member 4 | 5.3 | 1914285 | ||
| Ephx1 | epoxide hydrolase 1, microsomal | 2.0 | 3.2 | 95405 | |
| Hmox1 | heme oxygenase (decycling) 1 | 6.0 | 96163 | ||
| Mapk14 | mitogen-activated protein kinase 14 | 2.0 | 1346865 | ||
| Pik3cb | phosphatidylinositol 3-kinase, catalytic, beta polypeptid |
2.3 | 1922019 | ||
| Prdx1 | peroxiredoxin 1 | 2.9 | 3.1 | 99523 | |
| Raf1 | v-raf-leukemia viral oncogene 1 | 2.2 | 97847 | ||
| Sod2 | superoxide dismutase 2, mitochondrial | 1.7 | 98352 | ||
| Sqstm1 | sequestosome 1 | 3.1 | 107931 | ||
| Txnrd1 | thioredoxin reductase 1 | 2.1 | 1354175 | ||
| Xdh | xanthine dehydrogenase | 2.0 | 98973 | ||
| Signal Transduction | Fcrl1 | Fc receptor-like 1 | 3.5 | 3.9 | 2442862 |
| Lyn | Yamaguchi sarcoma viral (v-yes-1) oncogene homolog | −1.9 | 96892 | ||
| Rasgrp3 | RAS guanyl releasing protein 3 (calcium and DAG- regulated) |
3.9 | 4.4 | 3028579 | |
| Rit1 | Ras-like without CAAX 1 | 2.1 | 108053 | ||
| Small Molecule Biochemistry |
Cp | ceruloplasmin (ferroxidase) | −7.7 | −6.0 | 88476 |
| Slc7A2 | solute carrier family 7 (cationic amino acid transporter, y+ system), member 2 |
−5.2 | −5.2 | 99828 | |
| Cell Structure | Gsn | gelsolin | 2.5 | 95851 | |
| Stmn1 | stathmin 1 | 10.8 | 96739 | ||
| Tuba4 | tubulin, alpha 4 | 2.5 | 1095410 | ||
| Ubiquitin-Proteasome Pathway |
Fbxl17 | F-box and leucine-rich repeat protein 17 | 1.8 | 1354704 | |
| Fbxl20 | F-box and leucine-rich repeat protein 20 | 2.1 | 1919444 | ||
| Fbxo22 | F-box only protein 22 | 3.0 | 1926014 | ||
| Fbxo30 | F-box protein 30 | 2.0 | 1919115 | ||
| Fbxo31 | F-box protein 31 | 1.8 | 1354708 | ||
| Herc3 | hect domain and RLD 3 | 2.3 | 1921248 | ||
| Map1lc3b | microtubule-associated protein 1 light chain 3 beta | 2.0 | 1914693 | ||
| Psmc3ip | proteasome (prosome, macropain) 26S subunit, ATPase 3, interacting protein |
6.4 | 1098610 | ||
| Psmd12 | proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 |
2.0 | 1914247 | ||
| Rnf128 | ring finger protein 128 | 4.2 | 1914139 | ||
| Rnf167 | ring finger protein 167 | 1.9 | 1917760 | ||
| Ube2d3 | ubiquitin-conjugating enzyme E2D 3 (UBC4/5 | 2.4 | 1913355 | ||
| Ube2i | ubiquitin-conjugating enzyme E2I | 3.0 | 107365 | ||
| Ube2t | ubiquitin-conjugating | 7.1 | 1914446 | ||
| Ube4b | ubiquitination factor E4B, UFD2 homolog (S. | 1.8 | 1927086 | ||
| Uchl1 | ubiquitin carboxy-terminal hydrolase L1 | 4.4 | 103149 | ||
| Usp18 | ubiquitin specific protease 18 | −224.6 | 1344364 | ||
| Other | Arrdc3 | arrestin domain containing 3 | 7.0 | 2145242 | |
| Bcl2l11 | BCL2-like 11 (apoptosis facilitator) | 2.0 | 1197519 | ||
| Epsti1 | epithelial stromal interaction 1 (breast) | −2.1 | −10.6 | 1915168 | |
| Gabarapl1 | gamma-aminobutyric acid (GABA(A)) receptor- | 2.8 | 1914980 | ||
| Id1 | inhibitor of DNA binding 1 | −2.0 | 96396 | ||
| Ier3 | immediate early response 3 | −2.0 | 104814 | ||
| Ifi203 | interferon activated gene 203 | −3.7 | −5.2 | 96428 | |
| Klhl6 | kelch-like 6 (Drosophila) | −1.9 | −3.8 | 2686922 | |
| Map1d | methionine aminopeptidase 1D | 1.8 | 1.9 | 1913809 | |
| Ms4A6D | membrane-spanning 4-domains, subfamily A, | −2.3 | −3.9 | 1916024 | |
| Top2a | topoisomerase (DNA) II alpha | 13.2 | 98790 | ||
| Trim30 | tripartite motif-containing 30 | −7.2 | −31.8 | 98178 | |
| Uvrag | UV radiation resistance associated gene | −1.9 | −1.8 | 1925860 | |
| Zak | sterile alpha motif and leucine zipper containing | −1.9 | −4.0 | 2443258 | |
| Zbp1 | Z-DNA binding protein 1 | −5.7 | −69.4 | 1927449 | |
| Zbtb20 | zinc finger and BTB domain containing 20 | 3.6 | 1929213 | ||
| Zfp36 | zinc finger protein 36 | −1.9 | 99180 | ||
a
Genes altered ≥1.8-fold (p ≤ 0.01) up or down in LPS stimulated HNE-treated cells relative to LPS stimulated cells. Fold changes (FC) represent the average of three independent biological experiments.