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Human embryonic stem cell (hESC) colony under conventional culture conditions for 7 days of culture (left panel). Signs of differentiation such as a doughnut‐shaped colony structure and flattened cells in the middle of the colony (arrow) and on the periphery of the colony are evident. Representative hESC colony (right panel) cultured in media containing noggin (400 ng/ml) showing no signs of differentiation even after 13 days of culture.
