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. 2009 Jun 2;42(4):425–433. doi: 10.1111/j.1365-2184.2009.00616.x

Figure 2.

Figure 2

Reverse transcription–polymerase chain reaction analysis of control [irradiated mouse embryonic fibroblasts (iMEF) conditioned medium], noggin‐treated (iMEF conditioned medium containing noggin) and BMP4‐treated cells (iMEF conditioned medium containing BMP4). (a) Treatment with noggin (400 ng/ml) reduced expression of trophoblast differentiation markers. Cells treated with BMP4 (90 ng/ml) to induce differentiation towards trophoblast lineage expressed trophoblast markers (hTFAP2α, Hand1 and hCGβ) as did control cultures containing colonies undergoing spontaneous differentiation. GAPDH served as a loading control. (b) Transcripts for BMP2, BMP7 and BMP antagonist Gremlin remained unchanged across the treatment groups. Transcripts for noggin and chordin were not detected (data not shown).