Figure 7.

Effect of noggin on cell proliferation. Carboxyfluorescein succinimidyl ester (CFSE) was used to compare the degree of cell proliferation between cells exposed to noggin versus control cells. Cells were cultured on Matrigel or Matrigel supplemented with noggin. CFSE was added on day 4 post‐plating as described in the Materials and methods section and cells were cultured for an additional 7 days. Cells were then washed and analysed by FACS for CFSE intensity. Cells cultured with noggin had reduced levels of CFSE (shift to the left in the graph) indicative of an increased rate of proliferation. Data shown are representative of a set of four separate experiments. Treatment with noggin significantly (P < 0.01) increased the rate of cell proliferation (division index) as shown in the bar graph.