Table 1.
Sequence of forward and reverse primers used
| Markers | Forward primer | Reverse primer |
|---|---|---|
| Oct4 | CGTGAAGCTGGAGAAGGAGAAGCTG | CAAGGGCCGCAGCTTACACATGTTC |
| BMP4 | AGGAGCTTCCACCACGAAGAACAT | TTCAGTGGGCACACAACAGGCTTT |
| TFAP2α | TCCCTGTCCAAGTCCAACAGCAAT | TGGCAGGAAATTCGGTTTCGCACA |
| Hand1 | ATTAACAGCGCATTCGCGGAGTTG | TGGTTTAACTCCAGCGCCCAGACTT |
| hCGβ | ACGACTGAGTCTCTGAGGTCACTT | ACAGATGGTGGTGGTGACGGTGAT |
| GAPDH | TGAAGGTCGGAGTCAACGGATTTGGT | CATGTGGGCCATGAGGTCCACCAC |
| Flk1 | ACGCTGACATGTACGGTCTAT | GCCAAGCTTGTACCATGTGAG |
| Nodal | AGACATCATCCGCAGCCTACA | GACCTGGGACAAAGTGACAGTGAA |
| Nanog | ACCAGAACTGTGTTCTCTTCCACC | GGTTGCTCCAGGTTGAATTGTTCC |
| Cripto | CAGAACCTGCTGCCTGAATG | GTAGAAATGCCTGAGGAAAGC |
| Pax6 | AACAGACACAGCCCTCACAAACA | CGGGAACTTGAACTGGAACTGAC |
| Brachyury T | GTGACCAAGAACGGCAGGAGG | TGTTCCGATGAGCATAGGGGC |