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Western blot assay showed that the SIRT2 siRNA treatment led to a significant decrease in the SIRT2 levels of BV2 cells. The cells were transfected with SIRT2 siRNA for either 48 or 72 hrs, and subsequentlythe SIRT2 levels of the cells were determined by Western blot assay. N = 4-5. Data were collected from four independent experiments. *, p< 0.05; **, p< 0.01.
