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. 2014 Oct 11;6(3):166–171.

Figure 4.

Figure 4

Treatment of microglial BV2 cells with SIRT2 siRNA led to a significant increase in late-stage apoptosis of the cells, as assessed by FACS-based Annexin V/7-AAD staining. A. The FACS diagrams showed that SIRT2 siRNA induced an increase in the number of Annexin V+/7-AAD+ cells, assessed at 72 hrs after siRNA transfection. In the four fields of the original images from the flow cytometry-based study, the number of the dots indicates the number of Annexin V-/7-AAD- (the bottom-left field), Annexin V+/7-AAD- (the bottom-right field), Annexin V-/7-AAD+ (the top-left field), and Annexin V+/7-AAD+ cells (the top-right field), respectively. B. Quantifications of the results from the flow cytometry-based study indicated that SIRT2 siRNA led to a significant increase in the number of Annexin V+/7-AAD+ cells, assessed at 72 hrs after siRNA treatment. N = 3. Data were collected from three independent experiments. *, p< 0.05.