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. 2014 Oct 24;9(10):e109268. doi: 10.1371/journal.pone.0109268

Figure 6. ROS, OAT3, AhR, and NF-κB p65 are involved in IS-induced prorenin expression in vascular smooth muscle cells.

Figure 6

Serum starved HASMCs were pretreated with NAC (2.5 mmol/L) and DPI (10 µmol/L) for 30 min before incubation with IS (250 µmol/L) for 24 h (A, B). HASMCs were transfected with or without OAT3 siRNA (10 nmol/L), AhR siRNA (30 nmol/L) or p65 siRNA (10 nmol/L), and serum starved for 24 h, followed by incubation with IS (250 µmol/L) for 24 h. Cell lysates were immunoblotted using anti-OAT3, anti-AhR, anti-p65 and anti-prorenin antibodies (C–F). Mean±SE (n = 3). *p<0.05, **p<0.01 vs untreated group, #p<0.01 vs IS-treated group. Ctrl: control.