Figure 3. Comparison of the 1H-15N-HSQC spectra of f-2H, u-13C, 15N-labelled R1 in the absence (blue) and presence (red) of a saturating concentration of fractionally deuterated 3D7 PfAMA1104–442 at pH 7 and 40°C.
Some amide resonances (Ala3, Phe9, Ser10, Lys11, Phe12, Gly13, Ser14 and Arg15) of free R1 were broadened beyond detection at pH 7 and 40°C and their predicted resonances are indicated as black circles in the spectrum (prediction was made as described in the text). N/H indicates unassigned amide resonances of bound R1.