Figure 5. 1,10-PT suppressed the T. vaginalis-induced dissociation of the Bcl-xL/Bim and Mcl-1/Bim complexes in SiHa cells.

SiHa cells were treated as in Fig. 1A (A) or incubated with T. vaginalis, 1,10-PT-pretreated T. vaginalis ^†, or T. vaginalis and 1,10-PT simultaneously^‡ (B). Whole-cell lysates were subjected to immunoprecipitation (IP) using anti-Bim and anti-Bcl-xL antibodies. IP and input samples were resolved by SDS-PAGE and probed with the indicated antibodies. A total of 1% of the cell extract volume from each sample was used as input control.