Figure 1.
A. Immunoblotting analysis of DDX3X in parental PC9 cells, mock transfectants, and A-1 cells that were transfected with cDNA encoding DDX3X. B. Immunoblotting analysis of Sox2, KLF4, and c-Myc, in parental PC9 cells, mock transfectants, and A-1 cells. C. Analysis of anchorage-independent cell proliferation by phase-contrast microscopy. D. 1×105 PC9 parental cells or A-1 cells were cultured on 24-well plates for 3 days. Nonadherent cells were collected by gentle rocking in culture medium. The ratio of nonadherent cells was calculated based on the total number of adherent cells. Dead cells were excluded with trypan blue exclusion method. *P<0.05. Data are presented as the mean ± SD of three independent experiments.