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. 2014 Oct 24;9(10):e111285. doi: 10.1371/journal.pone.0111285

Figure 3. Treg level regulation by IL-2 and IL-2/anti-IL-2.

Figure 3

Peripheral (A) and spleen/lymph nodes (B) Tregs levels were evaluated at several intervals after IL-2 and IL-2/anti-IL-2 treatment and after LPS in both WT and P2×7−/− mice. In the case of IL-2, determination of circulating and tissue Tregs was done after 7 and 14 days from IL-2 that is the time potentially required to achieve a regulatory effect of the cytokine [21].; Tregs were also determined 3 days after LPS that means 17 days from IL-2 treatment. The time required for a regulatory effect of IL-2/anti-IL-2 is instead 5 days. Treg levels were accordingly determined at this time (i.e. 5 days after IL-2/anti-IL-2) and after 3 days from LPS corresponding to 8 days of treatment. * = p≤0.05, ** = p≤0.005, *** = p≤0.0005.