Figure 4. CD44^hiCD24^lo BT20 spheroid cells have a lower expression of DR4 in comparison to non-stem like cells.

(A) Confocal micrographs of FACS sorted CD44^hiCD24^lo cells from BT20 and MCF7 spheroids stained with FITC conjugated CD44 antibody and PE conjugated CD24 antibody (Scale bar  = 50 µm) (B) Bright field images of CD44^hiCD24^lo and non-stem like cells from BT20 and MCF7 spheroids (C) Flow cytometry histograms comparing the expression of death receptors in FACS sorted cells and monolayer cells.