| Cycle step | Temperature | Time | Number of cycles |
| Initial denaturation | 95 °C | 30 sec | 1 |
| Denaturation | 95 °C | 5 sec | 30 |
| Annealing | 60 °C | 10 sec | |
| Extension | 72 °C | 15 sec/kb | |
| Final extension | 72 °C | 5 min | 1 |
| 4 °C | hold |
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