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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: J Vasc Surg. 2014 Apr 24;62(2):464–470. doi: 10.1016/j.jvs.2014.02.053

Fig 4.

Fig 4

The effect of Hedgehog (Hh) signaling inhibition on Notch/transforming growth factor β (TGF-β)/α-actin messenger RNA (mRNA) expression in human aortic smooth muscle cells (HASMCs). Quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis of mRNA levels of Ptc-1, Gli-2, Notch 1, Hrt-2, α-actin, and TGF-β1 in HASMCs treated with cyclopamine (40 μM) for 24 hours. Data are normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and represent the mean ± standard error of the mean; n = 4.