The effect of Hedgehog (Hh) signaling inhibition on Notch/transforming growth factor β (TGF-β)/α-actin messenger RNA (mRNA) expression in human aortic smooth muscle cells (HASMCs). Quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis of mRNA levels of Ptc-1, Gli-2, Notch 1, Hrt-2, α-actin, and TGF-β1 in HASMCs treated with cyclopamine (40 μM) for 24 hours. Data are normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and represent the mean ± standard error of the mean; n = 4.