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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Genes Immun. 2014 Jul 3;15(7):440–448. doi: 10.1038/gene.2014.34

Figure 5.

Figure 5

The KIR2DL1 SNP is associated with decreased distal transcript expression. Purified NK cell RNA from purified donor NK cells was subjected to qRT-PCR with KIR2DL1-specific primers detecting distal (a) or proximal (b) transcripts. Expression relative to actin is shown on the Y-axis, and KIR2DL1/KIR2DS1 genotype of individual donors is listed on the x-axis. Donors were: lacking both KIR2DL1/KIR2DS1 (negative); two copies of wild-type KIR2DL1 (2Wt); one copy of Wt KIR2DL1 (1Wt); one Wt, one ZEB1-mutant KIR2DL1 copy (Wt/Mut); one copy of a mutated KIR2DL1 (1 Mut); and one copy of mutated KIR2DL1 and one copy of KIR2DS1 (Mut/2DS1). (c) Transcription start sites (TSS) mapped by 5’ RACE are shown for the 365 bp region immediately 5’ of the KIR2DL1 start codon ( ATG). TSS are indicated by an asterisk. The underlined sequences represent forward PCR primers used for the detection of distal and proximal transcripts in a and b .