Table 1.

Spectral counts of target proteins for five replicates of flow-through fractions (FFs) at 20 μL/min

Protein names	Replicate 1	Replicate 2	Replicate 3	Replicate 4	Replicate 5	Mean±SD
Alpha-2-macroglobulin	136	165	146	153	138	148±11.8
Serum albumin	2	–	1	1	1	1±0.5
Apolipoprotein A-I	4	7	2	4	4	4±2
Apolipoprotein A-II	2	2	2	2	3	2±0.5
Apolipoprotein B-100	95	112	107	76	95	97.0±13.9
Complement C3	–	1	3	3	7	4±2
Fibrinogen alpha chain	89	80	86	96	90	88±5.9
Fibrinogen beta chain	75	66	77	72	74	73±4.2
Fibrinogen gamma chain	53	38	46	49	49	47±5.6
Ig alpha-1 chain C region	2	4	3	4	4	3±0.9
Ig mu chain C region	10	10	12	10	12	11±1.1

Only target proteins identified in the FFs are listed