Figure 3. Elongated focal adhesions formation on collagen gels.
Fibroblasts (MKFs) show discrete, elongated focal adhesions on soft collagen gels with modulus of 104 Pa and 391 Pa (A-B) that are generally seen on very hard glass substrates (~70 GPa) (C). At least three independent experiments were performed per condition. (D-E) Quantitative results also suggest no statistically significant difference of FA size and number between each condition (Student’s t test, p > 0.1). Total n = 24 cells were analyzed.