Table 1.

Experimental design calendar for the application of sham signal or low magnitude mechanical stimulus (LMMS) to MDA-MB-231 breast cancer cells

D(-1)	D0	D1	D2	D3	D4	D5
○	○	X,†	X	X,†	X	X,†
D6	D7	D8	D9	D10	D11	D12
○	○	X	X,†	X	X	X,†
D13	D14	D15	D16	D17	D18	D19
○	○	X	X	X	X	X,†

X, LMMS/Sham; ○, Rest; ^†, Evaluation.

After plating cells on D(-2), LMMS or sham signal was applied starting from D1 until D19, 15 min/day, 5 days/week. Cultures were terminated on selected days for the evaluation of cellular indices.