Table 1.
Genes significantly modulated by BaP in individual organs (Welch’s t-test, p < 0.05) for at least three conditions
| Probe | Symbol | Gene name | EntrezID | M1* | M2 | M3 | F1 | F2 | F3 |
|---|---|---|---|---|---|---|---|---|---|
| Lung (target) | |||||||||
| H3018D10 | Actb | Actin, beta, cytoplasmic | 11461 | 1.310 | 1.280 | 1.290 | 1.020 | 1.010 | 1.080 |
| H3089D06 | Cav | Caveolae protein 1 | 12389 | 2.350 | 2.270 | 2.260 | 1.500 | 1.730 | 1.660 |
| H3110F10 | Ephx1 | Epoxide hydrolase 1, microsomal | 13849 | 1.424 | 1.696 | 1.658 | 2.220 | 2.382 | 2.910 |
| H3114C04 | Giyd2 | Giy-yig domain containing 2 | 75764 | 0.530 | 0.800 | 0.610 | 0.430 | 0.410 | 0.600 |
| H3125H11 | Hba-a1 | Hemoglobin alpha, adult chain 1 | 15122 | 0.597 | 0.687 | 0.487 | 0.502 | 0.442 | 0.580 |
| H3117D02 | Hbb-y | Hemoglobin y, beta-like embryonic chain | 15135 | 0.445 | 0.732 | 0.356 | 0.369 | 0.217 | 0.405 |
| H3139E01 | Hspa8 | Heat shock protein 8 | 15481 | 1.687 | 2.068 | 1.994 | 2.060 | 1.954 | 1.625 |
| H3097B08 | Pdia3 | Protein disulfide isomerase associated 3 | 14827 | 1.211 | 1.283 | 1.388 | 1.270 | 1.126 | 1.232 |
| H3126C02 | Rpl18 | Ribosomal protein l18 | 19899 | 1.350 | 1.400 | 1.490 | 1.040 | 1.010 | 0.980 |
| H3112G04 | Rps5 | Ribosomal protein s5 | 20103 | 1.310 | 1.290 | 1.350 | 0.970 | 0.860 | 1.000 |
| H3068A11 | Tmed5 | Transmembrane emp24 protein transport domain containing 5 | 73130 | 1.270 | 1.310 | 1.330 | 1.320 | 1.120 | 1.270 |
| Spleen (target) | |||||||||
| H3077H03 | Ccng1 | Cyclin g1 | 12450 | 1.342 | 1.362 | 1.665 | 1.359 | 1.564 | |
| H3114C04 | Giyd2 | Giy-yig domain containing 2 | 75764 | 0.443 | 0.393 | 0.513 | 0.484 | 0.203 | 0.383 |
| H3126A10 | Hba-a1 | Hemoglobin alpha, adult chain 1 | 15122 | 0.540 | 0.661 | 0.581 | 0.358 | 0.327 | 0.335 |
| H3134E01 | Hbb-y | Hemoglobin y, beta-like embryonic chain | 15135 | 0.813 | 0.742 | 0.495 | 0.542 | 0.334 | 0.564 |
| H3051C06 | Hspa5 | Heat shock 70kd protein 5 | 14828 | 1.061 | 1.359 | 1.433 | 1.047 | 1.241 | 1.522 |
| H3139E01 | Hspa8 | Heat shock protein 8 | 15481 | 1.297 | 1.540 | 1.567 | 1.937 | 1.784 | 1.705 |
| H3097B08 | Pdia3 | Protein disulfide isomerase associated 3 | 14827 | 1.126 | 1.224 | 1.388 | 1.161 | 1.204 | 1.425 |
| H3130D03 | Pip5k1c | Phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma | 18717 | 0.330 | 0.340 | 0.420 | 0.640 | 0.470 | 0.530 |
| H3139D02 | Tubb5 | Tubulin, beta 5 | 22154 | 0.649 | 0.650 | 0.716 | 0.744 | 0.619 | 0.725 |
| Forestomach (target) | |||||||||
| H3011E09 | Actb | Actin, beta, cytoplasmic | 11461 | 1.210 | 1.860 | 1.930 | 1.670 | 1.520 | 1.950 |
| H3089D06 | Cav | Caveolae protein 1 | 12389 | 0.570 | 0.630 | 0.570 | 0.910 | 0.700 | |
| H3126H01 | Hba - a1 | Hemoglobin alpha, adult chain 1 | 15122 | 0.210 | 0.240 | 0.150 | 0.510 | 0.470 | 0.300 |
| H3051C06 | Hspa5 | Heat shock 70kd protein 5 | 14828 | 1.332 | 1.837 | 2.287 | 1.716 | 1.687 | 1.803 |
| H3139E01 | Hspa8 | Heat shock protein 8 | 15481 | 1.570 | 2.382 | 2.752 | 1.674 | 1.956 | 2.153 |
| H3130D03 | Pip5k1c | Phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma | 18717 | 0.520 | 0.550 | 0.740 | 0.590 | 0.560 | 0.790 |
| H3126C02 | Rpl18 | Ribosomal protein l18 | 19899 | 1.510 | 1.370 | 1.640 | 0.950 | 1.030 | 1.160 |
| H3112G02 | Rps5 | Ribosomal protein s5 | 20103 | 1.320 | 1.270 | 1.440 | 1.040 | 1.050 | 1.360 |
| H3080H08 | Tmed5 | Transmembrane emp24 protein transport domain containing 5 | 73130 | 0.660 | 0.690 | 0.720 | 0.610 | 0.580 | 0.530 |
| H3018E11 | Tubb5 | Tubulin, beta 5 | 22154 | 0.588 | 0.624 | 0.576 | 1.117 | 1.115 | 1.098 |
| Liver (non-target) | |||||||||
| H3133B06 | Ccnd2 | Cyclin d2 | 12444 | 1.757 | 2.639 | 1.519 | 1.724 | 1.532 | 1.924 |
| H3125H11 | Hba-a1 | Hemoglobin alpha, adult chain 1 | 15122 | 0.170 | 0.420 | 0.490 | 0.490 | 0.280 | 0.290 |
| H3139E01 | Hspa8 | Heat shock protein 8 | 15481 | 0.935 | 1.592 | 1.354 | 1.870 | 2.606 | 2.456 |
| H3129E07 | Rpl17 | Ribosomal protein l17 | 319195 | 1.130 | 1.320 | 1.200 | 1.220 | 1.370 | 2.050 |
| Colon (non-target) | |||||||||
| H3139E01 | Hspa8 | Heat shock protein 8 | 15481 | 1.397 | 1.493 | 1.559 | 1.240 | 1.577 | 2.110 |
| H3139D02 | Tubb5 | Tubulin, beta 5 | 22154 | 1.951 | 2.106 | 2.216 | 1.318 | 1.717 | 1.749 |
| Glandular stomach (non-target) | |||||||||
| H3089D06 | Cav | Caveolae protein 1 | 12389 | 2.140 | 1.530 | 1.300 | |||
| H3126H01 | Hba-a1 | Hemoglobin alpha, adult chain 1 | 15122 | 0.610 | 0.340 | 0.310 | 0.310 | 0.250 | 0.240 |
| H3051C06 | Hspa5 | Heat shock 70kd protein 5 | 14828 | 1.316 | 1.780 | 1.294 | 1.253 | 1.661 | 1.165 |
| H3029C06 | Hspa8 | Heat shock protein 8 | 15481 | 1.242 | 1.303 | 1.046 | 1.568 | 1.480 | 1.266 |
| H3029F12 | Rpl17 | Ribosomal protein l17 | 319195 | 1.340 | 1.350 | 1.020 | 1.400 | 1.400 | 2.130 |
*Columns M1, M2, M3, F1, F2 and F3 show the relative fold-change values in 6 exposure conditions (M1-male 125, M2-male 250, M3-male 375, F1-female 125, F2-female 250 and F3-female 375 mg/kg b.wt/day. Expression ratios in bold text mean being significant (p < 0.05) by Welch’s t-test. The genes shown in bold text were subsequently validated by Taqman gene expression assay.