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. 2014 Oct 9;14:103. doi: 10.1186/s12935-014-0103-y

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© Cheung et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Metastatic potential of HCC21 cells in NOD/SCID mice. (A) Subcutaneous injection of HCC21 cells into NOD/SCID mice led to primary tumor development at the site of injection and secondary tumors in the peritoneal cavity (scale bar = 10 mm). (B) Histological analysis of patient’s tumor and adjacent non-tumor liver tissues, primary and secondary xenograft tumors established in NOD/SCID mice. Sections were stained with haematoxylin and eosin (H&E) (upper panel), AFP (middle panel) and rabbit Ig control (lower panel). (C) Undetectable protein expression of E-cadherin in HCC21 (passage 12) while HepG2 served as positive control.