Table 1.
Effects of holding in EH medium on mitochondrial distribution pattern of equine oocytes examined before or after IVM
| Treatment | Nuclear chromatin configuration | n. of analyzed oocytes | Small mitochondrial aggregates (SA) n. (%) | Perinuclear/pericortical (P/P) n. (%) | Abnormal n. (%) |
|---|---|---|---|---|---|
| EH | FN | 4 | 4 (100) | 0 (0) | 0 (0) |
| F/I | 2 | 2 (100) | 0 (0) | 0 (0) | |
| CC | 24 | 9 (38) | 11 (46) | 4 (17) | |
| PI/MI | 11 | 7 (64) | 4 (36) | 0 (0) | |
| IMM | FN | 16 | 6 (38) | 3 (19) | 7 (44) |
| I | 13 | 11 (84) | 2 (15) | 0 (0) | |
| CC | 14 | 7 (50) | 5 (36) | 2 (14) | |
| PI/MI | 0 | 0 (0) | 0 (0) | 0 (0) | |
| EH-IVM | MII | 34 | 9 (26)a | 23 (68)a | 2 (6) |
| IMM-IVM | MII | 46 | 25 (55)b | 18 (39)b | 3 (6) |
Legend: Homogenous/heterogeneously fluorescent nucleus (FN); Fibrillar/Intermediate (F/I); Condensed chromatin (CC); Prometaphase I/Metaphase I (PI/MI); Metaphase II (MII).
Chi square test with the Yates correction: between treatments and chromatin configurations: a vs. b (P <0.05).