Table 2.
Effects of holding in EH medium on energy/redox parameters of equine oocytes examined before or after IVM
| Treatment | Nuclear chromatin configuration | n. of analyzed oocytes | Mitochondrial activity | Intracellular ROS levels | Mitochondria/ROS colocalization |
|---|---|---|---|---|---|
| EH | FN | 4 | 387.42 ± 347.10a | 661.20 ± 404.12 | 0.40 ± 0.13a |
| IMM | 9 | 1248.63 ± 657.93b,* | 1330.73 ± 651.51 | 0.64 ± 0.18b | |
| EH | F/I | 2 | § | § | § |
| IMM | 13 | 273.23 ± 142.95 | 709.72 ± 423.16 | 0.56 ± 0.23 | |
| EH | CC | 20 | 545.15 ± 283.98 | 829.89 ± 336.91 | 0.63 ± 0.16 |
| IMM | 12 | 509.19 ± 242.27** | 882.61 ± 486.82 | 0.67 ± 0.14 | |
| EH | PI/MI | 11 | 653.80 ± 191.53 | 946.64 ± 561.87 | 0.71 ± 0.11 |
| IMM | 0 | § | § | § | |
| EH -IVM | POST-IVM MII | 32 | 1112.45 ± 494.10 | 1434.54 ± 686.00 | 0.76 ± 0.14 |
| IMM-IVM | 43 | 1372.64 ± 481.34 | 1982.04 ± 762.74 | 0.84 ± 0.13 |
Legend: Homogenous/heterogeneously fluorescent nucleus (FN); Fibrillar/Intermediate (F/I); Condensed chromatin (CC); Prometaphase I/Metaphase I (PI/MI); Metaphase II (MII). Mitochondrial activity and intracellular ROS levels are presented as MitoTracker and DCF fluorescence intensities expressed in Arbitrary Densitometric Units (ADU). Mitochondria/ROS colocalization is expressed as Pearson’s correlation coefficient. One-way ANOVA followed by Multiple Comparison Dunn’s method: a,b P <0.05; *, **P =0.004; § values for categories with fewer than 3 oocytes are not shown.