Table 4.
Effects of holding in EH medium at controlled room temperature (25°C) on confocal energy/redox parameters of equine oocytes
| Treatment | Nuclear chromatin configuration | n. of analyzed oocytes | Mitochondrial activity | Intracellular ROS levels | Mitochondria/ROS colocalization |
|---|---|---|---|---|---|
| EH | FN | 3 | 162.89 ± 101.19 | 172.93 ± 46.40 | § |
| IMM | 2 | § | § | § | |
| EH | F/I | 4 | 437.35 ± 298.64 | 377.13 ± 181.20 | 0.40 ± 0.32 |
| IMM | 9 | 623.01 ± 376.96a | 421.44 ± 164.87 | 0.38 ± 0.08 | |
| EH | CC | 20 | 547.82 ± 499.54 | 278.53 ± 179.28 | 0.50 ± 0.10 |
| IMM | 19 | 722.93 ± 390.31a | 377.92 ± 184.92 | 0.49 ± 0.19 | |
| EH | PI/MI | 0 | § | § | § |
| IMM | 3 | 1292 ± 192.36b | 643.67 ± 312.69 | 0.60 ± 0.10 | |
| EH | MII | 0 | § | § | § |
| IMM | 2 | § | § | § |
Legend: Homogenous/heterogeneously fluorescent nucleus (FN); Fibrillar/Intermediate (F/I); Condensed chromatin (CC); Prometaphase I/Metaphase I (PI/MI); Metaphase II (MII). Mitochondrial activity and intracellular ROS levels are presented as MitoTracker and DCF fluorescence intensities expressed in Arbitrary Densitometric Units (ADU). Mitochondria/ROS colocalization is expressed as Pearson’s correlation coefficient. One-way ANOVA followed by Multiple Comparison Holm-Sidak method: a,b P <0.05; §: values for categories with fewer than 3 oocytes are not shown.