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. 2014 Oct 10;7:713. doi: 10.1186/1756-0500-7-713

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© Padaria et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Proteomic analysis of T. aestivum pAPX gene . SDS-PAGE analysis representing the TapAPX protein expression in E. coli BL21 strain grown at different time periods after IPTG induction (A). Western blot analysis of TapAPX protein using Anti-His antibody showing its deduced band of 32 kDa (B). His-tag purification using Ni-NTA column. E- purified recombinant fusion TapAPX protein from E. coli BL21 (pET28a-TapAPX), M-Marker (C). PMF of the over-expressed APX protein using MALDI-TOF/TOF (D).