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(A) Amino acid substitutions in the pore regions (or “P-loops”) for six Nav in Thamnophis sirtalis. All sequences are shown relative to rat Nav1.1, with residues involved in TTX binding underlined. Substitutions putatively conferring TTX resistance are shown in red. (B) Locations of putative resistance-conferring substitutions in Nav1.4, 1.6, and 1.7.
