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. 2014 Sep 5;171(19):4478–4489. doi: 10.1111/bph.12800

Figure 2.

Figure 2

Clofoctol activates three UPR pathways in PC3 cells. (A and B) PC3 cells were treated with the indicated concentrations of clofoctol or thapsigargin (TG, 1 μM) for 24 h. RT-PCR analysis of XBP-1 mRNA was performed to observe the spliced form (XBP-1s) and unspliced form (XBP-1u) of XBP-1 in PC3 cells (A). Thapsigargin was used as a positive control compound for XBP-1 splicing. The RT-PCR products of XBP-1 mRNA were digested with PstI to further distinguish the XBP-1s from XBP-1u. XBP-1s does not contain a PstI digestion site, thus showing no digestion products (B). (C and D) PC3 cells were treated with various concentrations of clofoctol for 24 h (C). PC3 cells were treated with 20 μM clofoctol for indicated time points (D). The levels of phosphorylated and total proteins were assessed by Western blot analysis.