Figure 5.

Clofoctol inhibits in vivo translation. (A and B) Total proteins in PC3 cells were metabolically labelled with [³⁵S]-Met. The cells were then treated with various concentrations of clofoctol for 24 h (A) or treated with 30 μM clofoctol for indicated time points (B). The total proteins were resolved by the SDS-PAGE. ³⁵S-labelled proteins were visualized by autoradiography. Cells without treatment with [³⁵S]-Met ([³⁵S] free) were used as a negative control. (C) The luciferase mRNA was translated in vitro using a rabbit reticulocyte lysate system. The reaction mixture was treated with either clofoctol or cycloheximide (CHX, 10 μM) and the luciferase activity assay was conducted to assess the in vitro translation of luciferase mRNA. Cycloheximide was used as a positive control compound to inhibit in vitro translation. Data represent mean + 95% confidence interval from three independent experiments performed in triplicate (**P < 0.001 vs. DMSO).