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. 2014 Sep 5;171(19):4490–4503. doi: 10.1111/bph.12791

Figure 1.

Figure 1

Genistein potentiates iodide efflux by F508del- and A561E-CFTR after their rescue by low-temperature incubation. (A and B) Time courses of cumulative iodide efflux from BHK cells expressing wild-type, F508del- and A561E-CFTR. BHK-WT-CFTR cells were grown at 37°C, whereas BHK-F508del-CFTR and BHK-A561E-CFTR cells were grown at 37°C (A) or 27°C for 24 h before the experiment (B). During the periods indicated by the bars, cells were treated with forskolin (10 μM) and genistein (50 μM). (C) Effects of low temperature incubation on iodide efflux by F508del- and A561E-CFTR. Cumulative iodide efflux at 12 min is expressed as a percentage of that of wild-type CFTR. Data are means ± SEM (n = 4); *P < 0.05 versus wild-type CFTR; †P < 0.05 versus same construct cultured at 37°C.