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. 2014 Sep 5;171(19):4490–4503. doi: 10.1111/bph.12791

Figure 6.

Figure 6

F508del- and A561E-CFTR exhibit a time-dependent loss of channel activity in intact cells studied at 37°C. The magnitude of CFTR-mediated iodide efflux elicited by BHK-WT-CFTR, BHK-F508del-CFTR and BHK-A561E-CFTR cells is shown for an 8 h period at 37°C. Prior to study, BHK-WT-CFTR cells were grown at 37°C, whereas BHK-F508del-CFTR and BHK-A561E-CFTR cells were grown at 27°C for 24 h. Values represent cumulative iodide efflux stimulated by forskolin (10 μM) and potentiated by genistein (50 μM) normalized to that measured at time t = 0 h, when cells expressing low temperature-rescued mutants were returned to 37°C. Data are means ± SEM (wild-type, n = 4–5; F508del-CFTR, n = 4–6; A561E-CFTR, n = 4–9); *P < 0.05 versus response of same cell line at t = 0 h.