Skip to main content
. 2014 Sep 5;171(19):4504–4519. doi: 10.1111/bph.12794

Figure 5.

Figure 5

Thrombin stimulates MAPK-dependent activation of AP-1 and NF-κB via PAR1. (A) Cells were pretreated with PPACK (1 μM) or SCH79797 (10 μM) for 1 h and then challenged with thrombin (3 U·mL−1) for the indicated time intervals. The phosphorylation of p38 MAPK, Erk1/2, and JNK1/2 were determined by Western blot. (B) Cells were pretreated with PPACK (1 μM), SCH79797 (10 μM), SB202190 (1 μM), U0126 (10 μM) or SP600125 (10 μM) for 1 h and then challenged with thrombin (3 U·mL−1) for the indicated time intervals. The phosphorylation of c-Jun and p65 were determined by Western blot (n = 3).